منابع مشابه
Ribonucleic Acid and Protein Synthesis in Mitotic Hela Cells
HeLa cells arrested in mitosis were obtained in large numbers, with only very slight interphase cell contamination, by employing the agitation method of Terasima and Tolmach, and Robbins and Marcus. Protein synthesis and RNA synthesis were almost completely suppressed in mitotic cells. Active polyribosomes were nearly absent in mitotic cells as compared with interphase cells treated in the same...
متن کاملRibonucleic acid and protein synthesis in Rhizophlyctis rosea zoospores.
LéJohn, Herbert B. (Purdue University, Lafayette, Ind.), and James S. Lovett. Ribonucleic acid and protein synthesis in Rhizophlyctis rosea zoospores. J. Bacteriol. 91:709-717. 1966.-The uniflagellate zoospores of Rhizophlyctis rosea display active motility and a high endogenous respiratory metabolism, but neither growth nor net ribonucleic acid (RNA) or protein synthesis can be measured by ord...
متن کاملEscherichia coli GENERAL EFFECTS ON RIBONUCLEIC ACID AND PROTEIN SYNTHESIS
In trimethoprim-inhibited RCStr strains of Escherichia coli, the expression of the RC control of stable RNA synthesis arose primarily from a decrease in the intracellular concentrations of glycine and methionine, and not from inhibition of the initiation ofnew protein chains. In non-supplemented cultures, experiments with rifampicin showed that the immediate response to the addition of trimetho...
متن کاملProtein and Ribonucleic Acid Synthesis during Germination of Uredospores.
Existing evidence suggests that there is no net synthesis of protein during germination of spores of some obligate parasites. The problem was reinvestigated with enzymatic hydrolysis, rather than acid hydrolysis or solubility properties, to estimate changes in protein and RNA. Procedures are detailed for the use of uniformly labeled (14)C-uredospores for the isolation and quantitative assay of ...
متن کاملA soluble ribonucleic acid intermediate in protein synthesis.
The cell-free rat liver system in which Cl*-amino acids are incorporated irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine triphosphate, guanosine dior...
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ژورنال
عنوان ژورنال: Progress of Theoretical Physics Supplement
سال: 1961
ISSN: 0375-9687
DOI: 10.1143/ptps.17.108